The target chromosome to be cut is not cut; (b) the arrow indicates the cutting path, indicating that the target chromosome has been cleaved and separated; (c) the arrow indicates the blank space left after the target chromosome is cut and collected, indicating the target chromosome When the plant chromosome has been cut by the viscous Eppen-light microdissection system, it is possible to clearly determine whether the cut target chromosome is cleaved, separated and collected.
2.2 DOP-PCR microamplification of a single chromosomal DNA was collected from the collected Asian cotton single chromosome and Asian cotton genome dof cap. From the diagram, the first round/DOFPCR micro-expansion was performed using SLuCUT automatic shin N>AS and sterile water 丨ts, and the first round of DOP-PCR amplification products was used, followed by the second round of DOP-Sepharose electrophoresis. See the electropherogram.
PCR micro-amplification, using the first round and the second round of micro-amplification products to perform DOI-PCR micro-amplification product electrophoresis map 1 by cutting 1 Asian cotton single chromosome 1 point 1 away from Jing collection 1ishingHouse. From (a) It can be seen that in the 2-11 lane (single cotton single chromosome), the DNA product was observed in most of the lanes and the 12th lane (positive control), while no DNA product was observed in lane 1 (negative control water); DNA products were visible in lanes 211 and 12, while no DNA product was observed in lane 1, indicating that the DNA product observed in lane 211 was likely to be from the cut and collected Asian cotton. A single chromosomal DNA also verified the DOP-PCR micro-expansion condition for the micro-DNA expansion of Asian cotton.
2.3 Verification of a single chromosome amplification product Agarose electrophoresis was carried out on a single micro-amplification product of Asian cotton single chromosome DNA, Asian cotton genomic DNA and sterile water, followed by Southern hybridization, and the Southern hybridization map is shown.
The Southern hybridization map was visualized. In the 311 lane and the positive control lane 12 (Asian cotton genomic DNA), the micro-amplification products of Asian cotton single chromosomal DNA and Asian cotton genomic DNA were stronger than the Asian cotton genomic DNA probe, respectively. Hybridization signal, while the hybridization signal of the Asian cotton genomic DNA probe was not observed in the negative control lane 1 (sterile water), indicating that the single chromosomal DNA cut and collected has homology with the Asian cotton genomic DNA. Furthermore, it was shown that the DNA of the single chromosome cut and collected was from Asian cotton genomic DNA. DOP-PCR micro-amplification, verified by agarose electrophoresis and Southern hybridization, fully demonstrated that the SLuCUT automatic laser microdissection system can be used simply, quickly and efficiently. Complete micro-cutting, micro-separation and micro-collection of plant chromosomes.
3 Discussion SLuCUT automatic laser micro-cutting system (hereinafter referred to as cutting system) has the characteristics of simple operation, fast and accurate, light pollution, etc. It is mainly convenient to select the cutting path. The cutting system can draw various irregular shapes by mouse or A variety of drawing graphics are used to select the cutting target, so that the cutting target can be arbitrarily drawn, and the cut sample (target chromosome) is not degraded. The cutting system has a laser diameter of less than 1 laser energy, and the focusing and cutting speed is controlled by software. The laser is cut by cold laser. The laser does not directly contact the separated sample, and does not degrade DNA, RNA and protein due to thermal effects. The viscous collection tube cap adheres to the separated membrane and the sample extraction process does not require laser bombardment, so there is no damage to the sample and the contamination is light. Throughout the operation process, the movement of the cutting system stage, the arbitrary drawing of the cutting path, the measurement of the cutting length, the focusing, the laser energy, the cutting speed, the photographing, saving, collecting, etc. are all completed by the easy-to-use UVCUT software. Therefore, the operation is simple and easy to grasp; but the microscopic examination of the cells is inconvenient, because the cutting system controls the stage to move up and down and left and right by moving the mouse, and moving the mouse is far less convenient than controlling the movement of the stage by the rotating screw of the ordinary microscope. Therefore, in order to save time, the ideal cells that have been microscopically examined must be marked under a normal microscope for searching when cutting.
Separation and expansion of a single chromosome makes it possible to narrow down some genetic studies from whole genome levels to a single chromosome level. Therefore, since the birth of chromosome micro-separation and micro-expansion technology, it has been widely concerned worldwide. Because different chromosome libraries can be selected for different purposes and uses, which is much more efficient than the entire genome, the cutting system will become a favorable weapon for gene mapping and gene cloning in cytogenetics and molecular biology. 3. With the mobile phone as the transmitter, the receiving antenna is a single wire, and the induced current received by the antenna is amplified by the amplifier to drive the diode to emit light. When the mobile phone talks or hangs up, the intensity of the induced current is judged by the degree of light emitted by the diode. Through this experiment, students can visually observe the formation of induced current and the process of transmission amplification, and the influence of different placement states of the antenna on the induced current intensity.
Conclusion of the experimental principle Starting from the condition that the electric field is the current of the conductor, it is analyzed that the DC current can only be transmitted in the closed loop in order to maintain a constant electric field; the induced current can be transmitted in the conductor structure of any shape, as long as the inside of the conductor is sometimes Variable electric field. Further comparison of DC current and induced current is summarized, and the similarities and differences between the two are summarized.
Finally, an experiment to demonstrate the conduction induced current in a non-closed conductor is proposed to deepen the understanding of the induced current.
we have pure bronze flange and bi-metallic flange,we also produce nibco flange,the material is CC491K and CC499K, Bronze Flange ,bi-metallic flange,nibco bronze flange,gunmetal flange
Bronze Flange
bronze flange,bi-metallic flange,nibco bronze flange,gunmetal flange
Taizhou Runde Company , https://www.smartfittings.com